Development and Characterization of Microsatellite Primers inGeranium carolinianum(Geraniaceae) with 454 Sequencing
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چکیده
منابع مشابه
Development and characterization of microsatellite primers in Geranium carolinianum (Geraniaceae) with 454 sequencing1
UNLABELLED PREMISE OF THE STUDY Microsatellite primers were developed for Geranium carolinanum, a North American winter annual herb, for use in population genetic analyses. • METHODS AND RESULTS Genomic DNA enriched for repeat-containing fragments was sequenced on the Roche 454 Titanium platform, resulting in 470 primer pairs developed from 1115 microsatellite-containing sequences. A subse...
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Osmanthus fragrans (Oleaceae) is an evergreen shrub or small tree that grows in south China. In this study, Roche 454 FLX+ sequencing combined with the magnetic bead enrichment method was used to isolate microsatellite markers from the genome of O. fragrans. A total of 1471 microsatellites that contained enough flanking sequences for primer pair design were identified from 89,633 raw sequencing...
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PREMISE OF THE STUDY Microsatellite markers from Primula sikkimensis (Primulaceae) were developed for testing deep lineage divergence and speciation events. METHODS AND RESULTS A total of 3112 microsatellites were identified from 61,755 unique reads though 454 pyrosequencing technology. Twenty-nine microsatellite loci were selected for PCR amplification and polymorphic analyses. Among the 29 ...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2013
ISSN: 2168-0450
DOI: 10.3732/apps.1300006